Plasmid Preparation:Article Title: Generalized Strategy for Engineering Mammalian Cell-Compatible RNA-Based Biosensors from Random Sequence Libraries.
Article Snippet: .. The V21 CS2+ RFPbb plasmid (gift from Randall Moon; Addgene plasmid 17112) was PCR amplified with Phusion polymerase using primers RFPbb_fwd and FRPbb_rev (35 cycles, denaturing: 98 °C for 10 s, annealing: 65 °C for 10 s, extension: 72 °C for 150 s). .. The pAV-U6+27-TornadoBroccoli plasmid (gift from Samie Jaffrey; Addgene plasmid 124360) was PCR amplified with Phusion polymerase using primers TBins_fwd and TBins_rev (35 cycles, denaturing: 98 °C for 10 s, annealing: 64 °C for 10 s, extension: 72 °C for 30 s).
Article Title: Generalized strategy for engineering mammalian cell-compatible RNA-based biosensors from random sequence libraries
Article Snippet: .. The V21 CS2+ RFPbb plasmid (gift from Randall Moon; Addgene plasmid 17112) was PCR amplified with Phusion polymerase using primers RFPbb_fwd and FRPbb_rev (35 cycles, denaturing: 98°C for 10 seconds, annealing: 65°C for 10 seconds, extension: 72°C for 150 seconds). .. The pAV-U6+27-Tornado-Broccoli plasmid (gift from Samie Jaffrey; Addgene plasmid 124360) was PCR amplified with Phusion polymerase using primers TBins_fwd and TBins_rev (35 cycles, denaturing: 98°C for 10 seconds, annealing: 64°C for 10 seconds, extension: 72°C for 30 seconds).
Polymerase Chain Reaction:Article Title: Generalized Strategy for Engineering Mammalian Cell-Compatible RNA-Based Biosensors from Random Sequence Libraries.
Article Snippet: .. The V21 CS2+ RFPbb plasmid (gift from Randall Moon; Addgene plasmid 17112) was PCR amplified with Phusion polymerase using primers RFPbb_fwd and FRPbb_rev (35 cycles, denaturing: 98 °C for 10 s, annealing: 65 °C for 10 s, extension: 72 °C for 150 s). .. The pAV-U6+27-TornadoBroccoli plasmid (gift from Samie Jaffrey; Addgene plasmid 124360) was PCR amplified with Phusion polymerase using primers TBins_fwd and TBins_rev (35 cycles, denaturing: 98 °C for 10 s, annealing: 64 °C for 10 s, extension: 72 °C for 30 s).
Article Title: Generalized strategy for engineering mammalian cell-compatible RNA-based biosensors from random sequence libraries
Article Snippet: .. The V21 CS2+ RFPbb plasmid (gift from Randall Moon; Addgene plasmid 17112) was PCR amplified with Phusion polymerase using primers RFPbb_fwd and FRPbb_rev (35 cycles, denaturing: 98°C for 10 seconds, annealing: 65°C for 10 seconds, extension: 72°C for 150 seconds). .. The pAV-U6+27-Tornado-Broccoli plasmid (gift from Samie Jaffrey; Addgene plasmid 124360) was PCR amplified with Phusion polymerase using primers TBins_fwd and TBins_rev (35 cycles, denaturing: 98°C for 10 seconds, annealing: 64°C for 10 seconds, extension: 72°C for 30 seconds).
Amplification:Article Title: Generalized Strategy for Engineering Mammalian Cell-Compatible RNA-Based Biosensors from Random Sequence Libraries.
Article Snippet: .. The V21 CS2+ RFPbb plasmid (gift from Randall Moon; Addgene plasmid 17112) was PCR amplified with Phusion polymerase using primers RFPbb_fwd and FRPbb_rev (35 cycles, denaturing: 98 °C for 10 s, annealing: 65 °C for 10 s, extension: 72 °C for 150 s). .. The pAV-U6+27-TornadoBroccoli plasmid (gift from Samie Jaffrey; Addgene plasmid 124360) was PCR amplified with Phusion polymerase using primers TBins_fwd and TBins_rev (35 cycles, denaturing: 98 °C for 10 s, annealing: 64 °C for 10 s, extension: 72 °C for 30 s).
Article Title: Generalized strategy for engineering mammalian cell-compatible RNA-based biosensors from random sequence libraries
Article Snippet: .. The V21 CS2+ RFPbb plasmid (gift from Randall Moon; Addgene plasmid 17112) was PCR amplified with Phusion polymerase using primers RFPbb_fwd and FRPbb_rev (35 cycles, denaturing: 98°C for 10 seconds, annealing: 65°C for 10 seconds, extension: 72°C for 150 seconds). .. The pAV-U6+27-Tornado-Broccoli plasmid (gift from Samie Jaffrey; Addgene plasmid 124360) was PCR amplified with Phusion polymerase using primers TBins_fwd and TBins_rev (35 cycles, denaturing: 98°C for 10 seconds, annealing: 64°C for 10 seconds, extension: 72°C for 30 seconds).
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